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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cancer Res. 2013 Jul 9;73(17):5347–5359. doi: 10.1158/0008-5472.CAN-13-0087

Figure 5. MSC-expressed CD44 acts as a transcriptional regulator under tumor conditioned mediain vitro.

Figure 5

(A) Protein expression of Twist is increased (p<0.05) in MSC exposed to TCM compared to serum free medium by confocal microscopy. (B) By western blot, sh44MSC showed a decrease in Twist protein expression compared to shNS MSC after 30 min or 24 h of TCM. (C) By real-time PCR, shCD44MSC showed a decrease in Twist expression similar to shNS MSC treated with s44 antagonist or MMP inhibitor. Y axis is expression fold change. (D) After 30 min TCM, CD44 association with the Twist promoter increased by 3.5-fold. Furthermore, Stat3, acetyl-Stat3 and p300 association with the Twist promoter also increased.