(A) Protein expression of Twist is increased (p<0.05) in MSC exposed to TCM compared to serum free medium by confocal microscopy. (B) By western blot, sh44MSC showed a decrease in Twist protein expression compared to shNS MSC after 30 min or 24 h of TCM. (C) By real-time PCR, shCD44MSC showed a decrease in Twist expression similar to shNS MSC treated with s44 antagonist or MMP inhibitor. Y axis is expression fold change. (D) After 30 min TCM, CD44 association with the Twist promoter increased by 3.5-fold. Furthermore, Stat3, acetyl-Stat3 and p300 association with the Twist promoter also increased.