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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cancer Res. 2013 Jul 9;73(17):5347–5359. doi: 10.1158/0008-5472.CAN-13-0087

Figure 6. Ectopic expression of Twist in CD44 knockdown MSC partially restores phenotype under tumor conditioned mediain vitro.

Figure 6

Validation of HA-tagged, ectopic Twist expression by (A) RT-PCR and (B) western blot. (C) Transwell migration assay showed that ectopic expression of Twist in sh44MSC increased the migration capacity 2-fold toward TCM and that this effect was not inhibited by oHA. (D) Ectopic Twist expression in sh44MSC showed an increase in angiogenesis-associated growth factor expression by RT-PCR compared to sh44MSC. Fold changes relative to shNSMSC expression levels. (E) HUVEC tube formation potential was significantly decreased (p<0.05) when conditioned with medium from shCD44MSC compared to medium from naïve MSC or shNS MSC but ectopic Twist expression restored pro-angiogenic potential in sh44MSC. Graph represents the number of branch points counted per image.