Figure 7.

SERS spectrum of the anti-influenza aptamer (1000 nM) - PEG spacer (100 nM) complex on a Ag nanorod substrate, (b) anti-influenza aptamer complex and binding buffer blank control, (c) anti-influenza aptamer complex and RSV (10⁵ PFU/mL) negative control, (d) anti-influenza aptamer complex and nucleoproteins from A/Uruguay, (e) anti-influenza aptamer complex and nucleoproteins from A/Brisbane, and (f) anti-influenza aptamer complex and nucleoproteins from B/Brisbane. Virus concentrations in (d) – (f) were adjusted to 1μg/mL (relative to HA content). Each spectrum shown is an average of 10 individual spectra for each particular sample. The dashed vertical lines in (a), (b), and (c) indicate the characteristic oligonucleotide bands for the influenza aptamer and its controls. The dashed vertical lines in (d), (e), and (f) indicate the oligonucleotide bands that changed after binding of the nucleoproteins. Asterisks indicate the presence of new bands in the aptamer complex corresponding to binding of the protein target.