Table 1.

Average membrane parameters

	Chol	4Chol	PC-Chol50	4PC-Chol50
Area (Å2)	38.0 ± 0.1		48.4 ± 0.5/PCa	48.4 ± 0.2/PC
	38.0 ± 0.2b	37.9 ± 0.1	38.0/Chold	38.0/Chold
	~37.0c
C-C dist. (Å)	32.3 ± 2.6	32.4 ± 2.8	34.4 ± 2.8	34.6 ± 2.9
	32.3 ± 2.8b
	30.5 ± 3.7c
P-P dist. (Å)			45.7 ± 2.7a	45.7 ± 2.9
Smol	0.61 ± 0.01	0.61± 0.01	0.70 ± 0.01	0.69±0.01
	0.61 ± 0.01b
Tilt (°)
C3-C17	8.0; (10.7 ± 5.8) 8.3b; (11.0± 6.1)b	7.8 (10.4 ± 5.6)	9.1 (12.3 ± 6.9)	9.3 (12.5 ± 7.1)
C17-C25	13.4; (20.9 ± 12.5) 12.8b; (20.8 ± 12.7)b	13.3 (20.4 ± 11.8)	12.6 (17.3 ± 10.1)	12.3 (17.3 ± 10.0)
#H-bonds/Chol	2.29 ± 0.05	2.29 ± 0.03	1.68 ± 0.08	1.70 ± 0.04
	2.29 ± 0.05b
MSD(10 ns) (Å2)	5.92 ± 1.2	5.95± 2.7	13.0 ± 4.8/Chol	13.4 ± 4.3/Chol
	7.37 ± 3.3b		18.5 ± 5.0/PC	20.0 ± 4.8/PC

Average values of the surface area available to the lipid (Area); distance between average positions of carbon C3 or phosphorus P atoms in the two leaflets of a bilayer (C-C dist.; P-P dist.); molecular order parameter, Smol, for the cholesterol hydrocarbon chains; tilt angle (Tilt) of the cholesterol rings (C3-C17) and chains (C17-C25); and number of Chol-water H-bonds per Chol (#H-bond/Chol); together with values of mean square displacement after 10 ns (MSD), in the Chol (Chol-2), and POPC-Chol50 bilayers as well as in the 4Chol and 4POPC-Chol50 bilayers. The errors are standard deviation estimates, except for Smol, where they are standard error estimates. For tilt, the most probable, and, in parentheses, the average and standard deviation values are given. For comparison, some values for the Chol crystal bilayers are also given.

^aFrom Ref. ¹⁹

^bChol-2 bilayer

^cChol crystal bilayer (anhydrous crystal)²⁰

^dThe value of A_Chol was fixed to the value obtained from the pure Chol bilayers