Figure 5.
Interactions established between Gal-3 and laminin or fibronectin contribute to migration of monocytes and macrophages. Polycarbonate filters of a Transwell System were coated with laminin or fibronectin as described in Section 2 and used to assay cell migration induced by soluble hrGal-3 (0.01 µM). Monocytes or macrophages (5 × 104 cells) were added into inserts and allowed to migrate for 40 min at 37°C. Medium was used as negative control and soluble or immobilized hrGal-3 as positive. The number of migrating cells was counted in five fields for each condition assayed in triplicate. Data are reported as mean of cells per field ± S.E.M. sGal3, soluble Gal3; iGal3, immobilized Gal3; Lam, laminin; Fibr, fibronectin. *P < 0.05 compared to iGal3 assayed with monocytes and # P < 0.05 compared to iGal3 assayed with macrophages. Results are representative from two independent assays.