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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2013 May 14;63:115–125. doi: 10.1016/j.freeradbiomed.2013.05.010

Fig. 5. Decreased activation of PARP-1 by OGG1 polymorphisms.

Fig. 5

(A) PARP-1 poly(ADP-ribosyl)ation reactions were performed by incubating the GST-tagged WT or polymorphic recombinant proteins with (+) PARP-1 for 10 minutes prior to the addition of the PARP-1 cofactors NAD+ and activated DNA. Reactions without PARP-1 (−) were a negative control. Reactions were incubated further for 30 minutes, and analyzed on a 12% polyacrylamide gel and immunoblotted with anti-PAR antibodies and anti-GST antibodies as a protein loading control. The polymorphic OGG1 proteins are unable to activate PARP1 above the background level shown in the GST lane. (B) The relative amount of PAR was quantified from immunoblots and normalized to the amount of GST protein. This value was then normalized to WT OGG1. The histogram represents the mean ± S.E.M. from three independent experiments. *p<0.05, **p<0.01, comparing WT and variant forms of OGG1 using Student’s t-test.