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. 2013 Aug 21;18(8):086012. doi: 10.1117/1.JBO.18.8.086012

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© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)

0091-3286/2013/$25.00 © 2013 SPIE

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Fig. 11 — Concurrent multispectral fluorescence SFE imaging of the BE phantom at 30-Hz frame rate. (a–c) Green, red, and combined display channels before applying CRS algorithm. (b) The FL-only targets exhibited erroneous fluorescence signal in the red channel. (c) Green FL appeared with a subtle yellow shade by the addition of red channel bias, due to FL emission cross-talk with the red dye channel. (d–f) Green, red, and combined display channels after CRS algorithm was applied. (e) The confounding FL red channel signal was strongly attenuated after CRS. (f) The combined image after CRS showed correctly rendered green FL, red PM, and orange two-dye images. All images were single-frame raw video outputs from SFE imaging (80-deg field of view, 500-line resolution).