Table 1.

Strengths and limitations for the three solutions.

Solutions	Strengths	Limitations	Comments
Image stitching	Avoids dye cross-talk. Can be used for extended review and less susceptible to fluorescence photobleaching. Can be saved as patient record.	Data processing is intensive and slow. Algorithms may not be robust enough to compensate for unpredictable motion and other factors for in vivo imaging.	Software and algorithm improvements are needed for real-time implementation.
Concurrent imaging using CRS algorithm	Reduces dye cross-talk. Real time Compatible with in vivo clinical scenario.	Prescan calibration is needed, and device settings must be maintained.	Best solution for the short term.
Frame sequential imaging	Avoids dye cross-talk. No prescan calibration is required. Near real-time multispectral fluorescence imaging. Compatible with in vivo scenario.