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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Med Entomol. 2013 Jan;50(1):171–178. doi: 10.1603/me12189

Fig. 4.

Fig. 4

qRT-PCR analysis of tissue-specific expression of defensin in house flies fed GFP-Staphylococcus aureus. Fold changes in mRNA expression of defensin (def) were calculated using REST-MCS software and calibrated to expression levels in teneral adults and the reference genes rps18 and tubulin. Log2 fold change in expression levels is shown for each tissue, time and biological replicate, and error bars represent SE of technical replicates. Asterisks denote significant upregulation of def from baseline expression levels (P < 0.05).