Figure 2.

(A) Layer-by-layer assembly of nanoparticles and chemical structures of the anionic and cationic lipids. (B) Transmission electron microscopic image of anionic nanoparticles (NP−) after uranyl acetate negative staining. (C) Transmission electron microscopic images of cationic nanoparticles (NP+) after uranyl acetate negative staining. Arrows indicate the multilayer. Scale bar, 50 nm. (D) Cytotoxic effect on IGROV-1 cancer cell line. (E) Comparison of cytotoxicity of cisplatin-loaded NP+ nanoparticles compared with free cisplatin in various human carcinoma cell lines.

Note: Adapted with permission from Khiati S, Luvino D, Oumzil K, Chauffert B, Camplo M, Barthélémy P. Nucleoside-lipid-based nanoparticles for cisplatin delivery. ACS Nano. 2011;5(11):8649–8655. Copyright (2011) American Chemical Society.⁷⁰