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. 2013 Aug 26;8:3271–3283. doi: 10.2147/IJN.S47597

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© 2013 Deng et al. This work is published by Dove Medical Press Ltd, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Ltd, provided the work is properly attributed.

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(A) Procedures involved in developing naked LPD and TLPD. (B) Thiolation of antibodies using Traut’s reagent and conjugation of thiolated antibody to maleimide groups on the DSPE-PEG-MAL. (C) Conjugation of DSPE-PEG-COOH with antibody.

Abbreviations: LPD, liposome-polycation-DNA complex; TLPD, targeted liposome-polycation-DNA; DSPE-PEG-MAL, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (ammonium salt); DSPE-PEG-COOH, distearoyl-N-(3-carboxypropionoyl poly(ethylene glycol) succinyl)phosphatidylethanolamine; EDC, 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide; DOTAP, 1,2-dioleoyl-3-trimethylammoniumpropane; NHS, N-hydroxy succinimide; DNA, deoxyribonucleic acid; DSPE-PEG, distearoyl-N-(monomethoxy poly(ethylene glycol) succinyl)phosphatidylethanolamine; Fab’, antigen-binding fragment; SH, hydrosulfide group; NH-, imido-group.