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. 2013 Aug 26;8:3271–3283. doi: 10.2147/IJN.S47597

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© 2013 Deng et al. This work is published by Dove Medical Press Ltd, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Ltd, provided the work is properly attributed.

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Cellular uptake of SMMC-7721 cells in vitro. After treatment of the cells at a FAM-siRNA concentration of 250 nM or 500 nM for 24 hours, mean fluorescence intensity was analyzed by flow cytometry. The data are shown as the mean ± standard deviation (n = 3).

Abbreviations: TLPD-FPM, targeted LPD when linker PEG was DSPE-PEG-MAL; TLPD-FPC, targeted LPD when linker PEG was DSPE-PEG-COOH; NTLPD-FPM, nontargeted control LPD when linker PEG was DSPE-PEG-MAL; NTLPD-FPC, nontargeted control LPD when linker PEG was DSPE-PEG-COOH; DSPE-PEG-MAL, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (ammonium salt); DSPE-PEG-COOH, distearoyl-N-(3-carboxypropionoyl poly(ethylene glycol) succinyl)phosphatidylethanolamine.