Fig. 3.

HSF1 induces polyubiquitin gene expression in cycling cells. (A) HSF1 and HSF2 bind to polyubiquitin (UBB and UBC) but not to monoubiquitin (RPS27A, UBA52) genes as shown by ChIP-seq. (B) Schematic illustration of the four ubiquitin coding genes in the human genome. (C and D) Heat stress leads to increased expression of polyubiquitin but not monoubiquitin genes in cycling cells, and the heat-induced ubiquitin expression is abolished in HSF1-depleted cells. The mRNA levels and statistical significances were analyzed as in Fig. 2.