Fig. 4.

HSFs control a broad range of cellular processes during stress. (A) Gene ontology analyses of HSF1 and HSF2 target genes in heat-treated cycling cells in respect to genomic region and to presence or absence of RNPII. Promoter is defined as −1,200 to +300 bp from the TSS, and gene body is coding sequence from +301 bp to the end of the gene. HSF1-specific target gene groups are indicated in red, HSF2-specific target gene groups are in green, and their shared target gene groups are in blue. (Lower) Fractions of HSF1 or HSF2 target sites that localize to RNPII-occupied sites. RNPII-containing sites in nontreated cycling cells are recovered from the ENCODE project (wgEncodeEH000529; Iyer Laboratory, University of Texas). (B) mRNA levels of HSF target genes during heat shock. mRNA levels and statistical significances were analyzed as in Fig. 2.