Fig. 1.

Thermal stability and overall structure of YajR. (A) Thermal denaturation curves of YajR variants. Thermal denaturation of full-length WT YajR, the G51W G69W, and D73R mutants, and truncation YajR-ΔYAM (i.e., residues 1−394 of WT) were measured using the blue fluorescence of the cpm dye (the left vertical axis), and that of the YAM-alone sample, which does not contain any Cys residue, was measured using the green fluorescence of Sypro orange (the right vertical axis). Raw data were analyzed with Prism (GraphPad). Multiple experiments were performed with good reproducibility and representative results are shown. (B) Cartoon representation of the YajR crystal structure. In the TM core, central helices (i.e., TMs 1, 4, 7, and 10), rocker helices (TMs 2, 5, 8, and 11), and supporting helices (TMs 3, 6, 9, and 12) are shown in green, yellow, and red, respectively. The short helix α6–7 is colored in blue and the YAM domain in orange.