Dynamic patterning of 5hmC at developmentally regulated genes. (A) 5hmC and 5mC tracks for two representative genes, Ncam1 and Egfr, with different expression paths across mOSN, GBC, and HBC cell types. Scale is reads per million. (B–F) 5hmC TSS profiles in mOSN, GBC, and HBC stages grouped into five developmental expression sets: (B) mOSN/GBC common (n = 2,577), (C) GBC/HBC common (n = 281), (D) mOSN-specific (n = 893), (E) GBC-specific (n = 557), and (F) HBC-specific (n = 1,566). Bars indicate a significant difference (Materials and Methods) between the profile of the correspondingly colored track and the HBC profile (B–D), the GBC profile (E), or the mOSN profile (F).