Fig. 7.

SetD8/GATA-1-corepressed genes differentially require the NuRD component, Mi2β. (A) Mi2β knockdown in G1E-ER-GATA-1 cells. (Left) Western blot to detect Mi2β protein levels. (Right) Real-time RT-PCR quantitation of mRNA of GATA-1 target genes that are FOG-1–sensitive, FOG-1–insensitive, and SetD8-sensitive (n = 5 ± SE) *P < 0.05. (B, Left) Venn diagram depicting the extent of overlap between GATA-1–repressed, Mi2β-repressed, and SetD8-repressed genes. (Right) Venn diagram depicting overlap of GATA-1-activated, Mi2β-activated, and SetD8-activated genes.