Fig. 3.

Cu down-regulated LRP1 and reduced Aβ binding in brain endothelial cells. (A) Representative Western blot analysis of LRP1. (B) The ¹²⁵I-Aβ42 binding in primary mouse endothelial cells treated with (+, 200 nM) and without Cu (−). (C) Representative LRP1 Western blot analysis. (D) The ¹²⁵I-Aβ42 binding with (200 nM, black column; 1 μM, gray column) and without Cu (clear column) in human brain endothelial cells (HBECs). (E) Representative LRP1 immunoblotting after immunoprecipitation with IgG or antiprion antibody in the presence (200 nM) and absence of Cu. (F) Relative levels of LRP1 from data as in E. β-Actin was the reference molecule for each Western blot analysis. Values are mean ± SEM, n = 3–5 independent experiments per group.