Fig. 2.

Experimental and model analysis of T. reesei (Tr) RUTC30 monoculture and bicultures of T. reesei RUTC30 with E. coli (Ec) K12 or E. coli NV3 pSA55/69. Error bars are ±SD for n = 3 technical replicates. (A) T. reesei RUTC30 monoculture on 20 g/L MCC. Modeling results are shown as smooth lines, and experimental results are shown as points. (B) RUTC30/K12 biculture on 10 g/L MCC. (C) RUTC30/NV3 biculture on 20 g/L MCC. E. coli data points are color-coded to indicate population fraction retaining plasmids pSA55/69 (error bars are shown in SI Appendix, Fig. S4C). (D) Fermentation product titers for NV3 monoculture (20 g/L glucose) vs. biculture experiment in C. Error bars are ±SD for n = 2 biological replicates. Acet, acetate; EtOH, ethanol; iButOH, isobutanol; Succ, succinate. (E) Local sensitivity analysis of the RUTC30/NV3 parameter set. The model was integrated with one-at-a-time ±25% perturbations to each parameter/IC (SI Appendix, section 2). Parameter effects were quantified by response coefficients, defined as , where is the percentage change in output Z and is the percentage change in parameter X. Response coefficients for and R_cel are shown for top 10 parameters (ranked by ), with the color code indicating the direction of the parameter/IC perturbation for the plotted response.