Fig. 1.

tsl functions in the PG independently of tor to regulate developmental timing. (A) tsl^Δ homozygotes pupariate ∼1 d later than controls. Weak ubiquitous expression (da-Gal4) of a UAS-tsl transgene results in a significant reduction (∼10 h, P < 0.001) in developmental delay compared with tsl^Δ. (B) tor^XR1 and tsl^Δ larvae exhibit a similar delay to pupariation compared with controls and a similar delayed transition from the L2 to the L3 stage. (C) Larvae deficient for both tor and tsl display a greatly extended delay in time to pupariation (83 h) compared with controls (P < 0.001). h AEL, hours after egg lay. Error bars represent ±1 SEM for all graphs. ***P < 0.001, **P < 0.01 from two-tailed t tests in all cases. n = 6 or greater for all means with no fewer than 52 individuals tested for each genotype.