Fig. 5.

Gross visualization of in vivo transfection. Organs from animals transfected with nanoparticles bearing the β-galactosidase gene are shown. The dark-blue 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) product accumulates in transfected regions. (A) Intrasplenic tumors transfected with CPS and adjacent normal spleen. (B) Intrasplenic tumors transfected with in vivo-JetPEI and adjacent normal spleen. (C) Normal lungs after transfection with CPS. (D) Normal lungs after transfection with in vivo-JetPEI.