Fig. 2.
The effect of LiCl treatment on restoration of appetite in AgRP neuron-ablated mice is transient. (A) Body weight of AgrpDTR/+ mice after 4 d of saline or LiCl treatment (0.25 M, 10 µL⋅g−1⋅d−1, i.p.; n = 6–8 per group). Injection of DT was initiated either 2 d (red line A and green line C traces) or 3 d (purple line B trace) after LiCl treatment was terminated. (B) Daily food intake was measured for the groups of mice described in A. Two-way, repeated-measures ANOVA revealed (i) no effect of LiCl treatment on either body weight or food intake from day −7 to day 0; (ii) a significant effect on both body weight and food intake at days 1–7 in saline-treated and LiCl-treated groups with a 2-d delay before DT treatment (*P < 0.05); and (iii) a significant effect of LiCl on both body weight and food intake from days 1–10 after 2-d vs. 3-d delay in DT treatment (*P < 0.05). Results are shown as means ± SEM.