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. 2013 Aug 14;110(36):E3445–E3454. doi: 10.1073/pnas.1303002110

Fig. 4.

Fig. 4.

ATSP-7041 activates p53 signaling in cancer cells in p53WT cell lines. (A) ATSP-7041 stabilizes p53 and elevates protein levels of p53 targets p21 and MDM2. Log-phase SJSA-1 and MCF-7 cells were incubated with 1.25, 2.5, 5.0, or 10 μM ATSP-7041 or 10 μM Nutlin-3a for 24 h, and cell lysates were analyzed by Western blotting. (B) ATSP-7041 shows dose-dependent induction of p53 target genes in p53WT but not in mutant p53 cell lines. Exponentially growing p53WT (SJSA-1, MCF-7) and p53 mutant (SW480, MDA-MD-435) cancer cell lines were incubated with 2.5, 5, or 10 μM ATSP-7041 or 10 μM Nutlin-3a for 24 h, and mRNA levels of p53 targets, p21, MDM2, and MIC-1 were measured by quantitative PCR and expressed as fold increase. (C) Viability of four p53WT (SJSA-1, MCF-7, HCT-116, and RKO) and two mutant p53 (SW480 and MDA-MB-435) cancer cell lines was determined after incubation with ATSP-7041 and expressed as percentage of controls ± SD.