Fig. 2.

Pulsed HDX (Left) and Western blotting (Right) results for three peptic peptides from Aβ₄₂. Aggregation was done at 25 °C and in the absence (solid triangles, circles, and diamonds) or presence of Cu²⁺ (hollow triangles, circles, and diamonds). Peptic peptides 1–19 (A), 20–35 (B), and 36–42 (C) are represented by triangles, circles, and diamonds, respectively. Dashed lines are fits using “linear fit” in OriginPro 8.5. Solid lines are fits using a modified F–W model in Mathcad. Native gel and Western blotting of the aggregated Aβ₄₂ samples (incubated at 25 °C for 48 h), in the absence (lane 1) or presence of Cu²⁺ (lane 2).