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. Author manuscript; available in PMC: 2014 May 10.
Published in final edited form as: Circ Res. 2013 Mar 15;112(10):1345–1354. doi: 10.1161/CIRCRESAHA.111.300581

Figure 3. The effects of apoA-I and HDL on palmitate-induced chemotactic factor gene expression are mediated by ABCA-1, ABCG-1 and SRB-1.

Figure 3

3T3-L1 adipocytes were transfected with a siRNA specific for ABCA-1 (A and B), ABCG-1 (C and D), SRB-1 (E and F) or a scrambled siRNA (negative control) as indicated. 24h later the siRNA was removed and the cells were cultured for a further 3 days. After that, the cells were pre-exposed to apoA-I (50µg/ml) or HDL (50µg protein/ml) for 6h, and then incubated with or without added palmitate (250µmol/L) for 24h. Total RNA was isolated and analyzed by multiplex real-time RT-PCR using Saa3-specific (A, C and E) or Mcp-1-specific (B, D and F) primers and normalized to Gapdh. *P < 0.001 vs. control media, **P < 0.001 vs. palmitate, #P < 0.001 vs. palmitate plus HDL or apoA-I.