3T3-L1 adipocytes were transfected with a siRNA specific for ABCA-1 (A and B), ABCG-1 (C and D), SRB-1 (E and F) or a scrambled siRNA (negative control) as indicated. 24h later the siRNA was removed and the cells were cultured for a further 3 days. After that, the cells were pre-exposed to apoA-I (50µg/ml) or HDL (50µg protein/ml) for 6h, and then incubated with or without added palmitate (250µmol/L) for 24h. Total RNA was isolated and analyzed by multiplex real-time RT-PCR using Saa3-specific (A, C and E) or Mcp-1-specific (B, D and F) primers and normalized to Gapdh. *P < 0.001 vs. control media, **P < 0.001 vs. palmitate, #P < 0.001 vs. palmitate plus HDL or apoA-I.