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. Author manuscript; available in PMC: 2014 May 10.
Published in final edited form as: Circ Res. 2013 Mar 15;112(10):1345–1354. doi: 10.1161/CIRCRESAHA.111.300581

Figure 5. HDL and apoA-I block the palmitate-induced NOX4 translocation via ABCA-1, ABCG-1 and SRB-1.

Figure 5

3T3-L1 adipocytes were transfected with a siRNA specific for ABCA-1, ABCG-1, SRB-1 or a scrambled siRNA (negative control) as indicated. 24h later siRNA was removed and the cells were cultured for a further 3 days. After that, the cells were pre-exposed to apoA-I (50µg/ml) or HDL (50µg protein/ml) for 6h, and then incubated with or without added palmitate (250µmol/L) for 24h. LRs were isolated and fractionated by ultracentrifugation using a detergent-free fractionation method. Proteins from OptiPre-gradient fractions were immunoblotted with anti-NOX4 and anti-caveolin-1 (CAV1) antibodis. PNS: the post-nuclear supernatant fraction.