Skip to main content
. Author manuscript; available in PMC: 2014 May 10.
Published in final edited form as: Circ Res. 2013 Mar 15;112(10):1345–1354. doi: 10.1161/CIRCRESAHA.111.300581

Figure 6. ApoA-I and HDL affect palmitate-induced NOX4-derived ROS generation via ABCA-1, ABCG-1 and SRB-1.

Figure 6

3T3-L1 adipocytes were transfected with a siRNA specific for ABCA-1, ABCG-1, SRB-1 or a scrambled siRNA (negative control) as indicated. 24h later the siRNA was removed and the cells were cultured for a further 3 days. After that, the cells were exposed to apoA-I (50µg/ml) or HDL (50µg protein/ml) for 6h, and then incubated with or without added palmitate (250µmol/L) for 24h. Cells were subjected to FACS analysis using CM-H2DCFDA. Results are plotted as counts (number of cells) on the vertical axis, versus DCF fluorescence intensity on the horizontal axis. Cells exposed to control media are shown in red and the peak value for cells exposed to 250µmol/L palmitate are indicated by the dashed lines. Cells exposed to the indicated treatments are shown in blue.