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. Author manuscript; available in PMC: 2014 May 10.

Published in final edited form as: Circ Res. 2013 Mar 15;112(10):1345–1354. doi: 10.1161/CIRCRESAHA.111.300581

ApoA-I^tg/tg and C57BL/6 control mice were fed chow or a high-fat, high-sucrose, cholesterol-containing (HFHSC) diet for 24 weeks (A–E; n=9). Epididymal fat was isolated and analyzed by real-time RT-PCR using Saa3, Mcp-1 (A), Mac2, F4/80 (B), Tnfα, Il1β, Il6 (D) and Nox4 (E)-specific primers and probes and normalized to Gapdh. Epididymal fat was isolated and analyzed by immunohistochemistry using a Mac-2 antibody (C), which detects murine macrophages. Tissues were photographed using microscopy (original magnification ×60. *P < 0.005 vs. chow, **P < 0.005 vs. C57BL/6 or LDLR^-/- in HFHSC.

ApoA-I^tg/tg and C57BL/6 control mice were fed chow or a high-fat, high-sucrose, cholesterol-containing (HFHSC) diet for 24 weeks (A–E; n=9). Epididymal fat was isolated and analyzed by real-time RT-PCR using Saa3, Mcp-1 (A), Mac2, F4/80 (B), Tnfα, Il1β, Il6 (D) and Nox4 (E)-specific primers and probes and normalized to Gapdh. Epididymal fat was isolated and analyzed by immunohistochemistry using a Mac-2 antibody (C), which detects murine macrophages. Tissues were photographed using microscopy (original magnification ×60. *P < 0.005 vs. chow, **P < 0.005 vs. C57BL/6 or LDLR^-/- in HFHSC.