Table 3. Primers used for in vitro dsRNA synthesis.
| Sequence Name | Primer Name | aPrimer sequence 5′ to 3′ | Product Size (bp) |
| Chitin Synthase | PCCHST7FPCCHST7R | GGATCCTAATACGACTCACTATAGGG CGTTGGCTGTGCACATTACT GGATCCTAATACGACTCACTATAGGG AAGCACCCCACCAACATAAG | 342 |
| V-ATPase | PCVATPT7FPCVATPT7R | GGATCCTAATACGACTCACTATAGGG AAGGTTGGCAGCCACATAAC GGATCCTAATACGACTCACTATAGGG CGAAAGCTCCTGGTATAGCG | 384 |
| Actin | PCActinT7FPCActinT7R | GGATCCTAATACGACTCACTATAGGG TCCGGTGATGGTGTATCTCA GGATCCTAATACGACTCACTATAGGG ATTTCTCGTTCGGCAGTTGT | 220 |
| GFP | GFPFGFPR | bCTAATACGACTCACTATAGG GCGGCCGCACGCGTGCTGAAGTCAAGTTbCTAATACGACTCACTATAGG GCGGCCGCCTTTTCGTTGGGATCTTTCG | 321 |
a
Underlined nucleotide sequence is the T7 RNA polymerase promoter sequence. A pair of gene specific primers with the T7 promoter sequence at the 5′ ends of both primers was used to amplify the desired templates. Amplified PCR products were used as templates for in vitro dsRNA synthesis.
b
Italic nucleotide sequence is the Not 1 restriction enzyme recognition sequence.