Figure 3. In vitro migration of cAT-MSC-Mock and cAT-MSC-IFN-β toward LMeC melanoma cells.

The migratory capacity of cAT-MSC-Mock and cAT-MSC-IFN-β were assessed by a modified transwell migration assay. LMeC cells (10⁵ cells/mL) were incubated in serum-free DMEM for 24 h, conditioned medium was collected and placed in the lower wells of the transwell plates. Serum-free medium served as negative control and medium supplemented with 10% fetal bovine serum was used as a positive control. (A) Representative photographs (magnification, ×200). (B) The cAT-MSC-Mock and cAT-MSC-IFN-β showed significant migration toward LMeC conditioned medium (CM) compared with the serum-free medium control. There was no significant difference in migration ability toward LMeC CM between cAT-MSC-Mock and cAT-MSC-IFN-β. Values represent the mean +SD. Data are representative of three independent experiments with similar results. * p<0.05, ** p<0.01, *** p<0.001.