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. 2013 Sep 9;8(9):e74897. doi: 10.1371/journal.pone.0074897

Figure 4. cAT-MSC-IFN-β directly inhibits the growth of LMeC melanoma cells in vitro.

Figure 4

LMeC melanoma cells were either cultured alone or co-cultured directly with CM-DiI-labeled cAT-MSC-Mock and cAT-MSC-IFN-β (the ratio tumor cells∶cAT-MSCs was 2∶1) for 72 h. (A) LMeC cells and cAT-MSCs stained by Hoechst, were examined with fluorescence microscopy. Scale bar  = 100 µm. (B) Numbers of diploid MSCs (red) and aneuploid LMeC melanoma cells (blue) were determined by flow cytometry and (C) cell count. cAT-MSC-IFN-β directly inhibited the growth of LMeC cells as compared with LMeC cells alone. Data are expressed as the percentage of cell number and compared with that of the control. Values represent the mean +SD. Data are representative of three independent experiments with similar results. * p<0.05 (Bonferroni's method for multiple comparisons).