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. 2013 Sep 9;8(9):e74678. doi: 10.1371/journal.pone.0074678

Figure 1. Agrobacterium-mediated transformation and regeneration of transgenic plants from immature embryo-derived callus of common wheat (Triticum aestivum L.).

Figure 1

a: Embryogenic calli (→) formed from immature embryos. b: Non-embryogenic calli (→) formed from immature embryos. c: Untransformed embryogenic calli (Control) on the regeneration medium supplemented with 25 mg/L G418. d: Shoot regeneration from Agrobacterium-infected embryogenic calli on the regeneration medium supplemented with 25 mg/L G418. e & f: Rooting of regenerated shoots on the rooting medium supplemented with 25 mg/L G418. g: G418-resistant plant in pot. h: Fertile G418-resistant plants in pot.