Figure 4. Inactivation of MNV on copper surfaces in the presence of quenchers D-mannitol or Tiron (A) or chelators EDTA or BCS (B) and to remove hydroxyl radical or superoxide, copper II or Cu I, respectively.

Approximately 5×10⁴ pfu MNV was inoculated onto metal surfaces in the presence of chelators or quenchers of reactive oxygen species and assessed for infectious virus using plaque assay as described in text. The results were compared to those obtained without chelators or quenchers to ascertain if there was a protective effect. No quenchers or chelators present is represented by white bars; D-mannitol (A) or EDTA (B) represented by diagonal striped bars; Tiron (A) or BCS (B) represented by cross hatched bars. No significant reduction of infectivity occurred in the presence of any quenchers or chelators on stainless steel surfaces (even though D-mannitol has been reported to interfere with HSV replication) (C and D respectively). Error bars represent ± SD and data are from multiple experiments.