Figure 3. One of the predicted ASF/SF2 binding sites downstream of HPV-16 SA3358 accounts for the majority of the enhancer activity.

(A). CAT protein levels produced in HeLa cells transfected with the indicated mutant pBELMCAT-derived plasmids [38]. CAT was monitored as described in Materials and Methods. Mean values and standard deviations are shown. Note the logarithmic scale. (B) RT-PCR with primers 757s and L1A on cDNA of cytoplasmic RNA extracted from HeLa cells transfected with the indicated plasmids. L1 and L1i mRNAs are indicated. M, molecular weight marker; GAPDH, cDNA amplified as internal control. (C, D) CAT protein levels produced in HeLa cells transfected with the indicated mutant pBELMCAT-derived plasmids [38]. CAT was monitored as described in Materials and Methods. Mean values and standard deviations are shown.