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Ni-NTA purified bR loaded nanodiscs were run over a HiLoad XK16/600 Superdex 200 column. Fractions containing bR loaded nanodiscs with a Stokes diameter of 12 nm were pooled and reinjected on an analytical Superdex 200 10/300GL column (inset) to verify stability of the bR nanodiscs. Solid and dotted lines show absorbance at 280 nm and 550 nm, respectively.
