Skip to main content
. 2013 Sep 9;8(9):e71982. doi: 10.1371/journal.pone.0071982

Figure 3. CTR1 silencing inhibits Cu entry.

Figure 3

A. Cu estimation by FACS: Histogram for Phen Green analysis was plotted with the mean fluorescent intensity obtained. Cu estimation by FACS, showed that treatment with Cu (# p = 0.04) increased the intracellular Cu levels (indicated by a decrease fluorescent intensity) and si 1 - 10 nM (*p = 0.02) decreased intracellular Cu levels in the presence of Cu, (indicated by an increase in fluorescent intensity). Ssi showed no significant effect and P 800 µM also showed significant inhibition (*p = 0.03) of Cu inside the cell. B. Cu estimation by AAS: Intracellular Cu levels increased significantly with Cu100 µM treatment (# p = 0.0005) and si 1 - 10 nM treatment decreased Cu levels (* p = 0.0008), even though P 800 µM also showed (* p = 0.01), a decrease in the Cu level statistically significant.