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. 2013 Sep 9;8(9):e72389. doi: 10.1371/journal.pone.0072389

Figure 1. Fluorescence resonance energy transfer probes (FRET) enable the distinction between bound and unbound probes upon hybridization of a pair of linear antisense ODNs to adjacent sequences on target mRNA.

Figure 1

When donor anchor is excited and both ODNs are fully complementary to target mRNA, a distance of 4