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. 2013 Sep 9;8(9):e72389. doi: 10.1371/journal.pone.0072389

Figure 7. Point mutation in Hras gene in DMBA/TPA treated cells.

Figure 7

(A) The A>T substitution causes the appearance of XbaI consensus site (B) XbaI digestion of Hras in MEF (left lane) and 308 cells (right lane). Two restriction fragments of 300 and 400 bp emerge in mutated Hras resulting from cleavage of full length 700 bp replicon in the newly formed XbaI restriction site. (C) Sequencing of Hras codon 61 reveals A>T transversion in of 308 cell line which is derived from early DMBA/TPA induced papillomas (ii). Mouse embryonic fibroblasts (MEFs) hold the typical A allele (i).