Table 1. Hras oligo probes sequences and modifications.

Oligonucleotide	Sequence (5′ –3′)
1. HRAS-anchor-AF488 PS	C*C*C*G*C*A*U*G*G*C*A*C*U*A*U*A-AF488
2. HRAS-T-TR-8mer -PS	TR-U*C*T*A*G*A*C*C
3. HRAS-A-TR-8mer -PS	TR -U*C*T*T*G*A*C*C
4. HRAS-T-AF594 -8mer-PS	AF594-U*C*T*A*G*A*C*C
5. HRAS-A-AF594 -8mer-PS	AF594-U*C*T*T*G*A*C*C
6. HRAS-anchor-AF488 PSEnd	SpC3-C*CCGCAUGGCACUAU*A-AF488
7. HRAS-T-TR -8mer-PSEnd	TR-U*CTAGAC*C
8. HRAS-A-TR -8mer-PSEnd	TR-UCTTGAC*C
9. HRAS-U-targ RNA	acagcaggucuagaagaguauagugccaugcgggaccagu
10. HRAS-A-targ RNA	acagcaggucaagaagaguauagugccaugcgggaccagu

Key: A,C,U/T,G –2′OMe RNA, a,c,u,g – RNA, A,C,T,G - LNA residues, Bold font - SNP site, TR – Texas Red, AF – Alexa Fluor, “*” - phosphorothioate (PS) internucleotide linkage, PSEnd- incidates phosphorothioate bonds only on terminal linakges of the molecule, targ- target sequence.