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. 2013 Sep 9;8(9):e73732. doi: 10.1371/journal.pone.0073732

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© 2013 Park et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Chemical structure of Ac-PAL-AMC. (b) Ac-PAL-AMC hydrolysis over time by purified constitutive proteasome (cross), purified immunoproteasome (closed circle), or purified immunoproteasome pre-treated with UK101 (open square). A linear increase was seen in immunoproteasome-containing wells only. (c) The rate of hydrolysis of Ac-PAL-AMC or Suc-LLVY-AMC in the presence of Panc-1 cell extract (10 µg). Hydrolysis of Ac-PAL-AMC was almost completely inhibited by UK101 pre-treatment (10 µM). Hydrolysis of Suc-LLVY-AMC was partially inhibited by UK-101 pre-treatment. Epoxomicin (Epx, 10 µM), a broadly-acting proteasome inhibitor, was used as a positive control.