Skip to main content
. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Curr Protoc Mol Biol. 2013 Jul;0 12:Unit–12.16. doi: 10.1002/0471142727.mb1216s103
Time Procedure
Day 1 Identify target sites using ZiFiT (Basic Protocol 1)
Day 1 Array digested units into plates
Day 2 Build TALE fragment (Basic Protocol 2)
Day 3 Clone TALE fragment (Basic Protocol 3)
Day 4 Start overnights and identify the clones that have fragments of the correct length using colony PCR (Basic Protocol 3)
Day 5 Sequence clones that have the correct length fragments (Basic Protocol 3)