Time | Procedure |
---|---|
Day 1 | Identify target sites using ZiFiT (Basic Protocol 1) |
Day 1 | Array digested units into plates |
Day 2 | Build TALE fragment (Basic Protocol 2) |
Day 3 | Clone TALE fragment (Basic Protocol 3) |
Day 4 | Start overnights and identify the clones that have fragments of the correct length using colony PCR (Basic Protocol 3) |
Day 5 | Sequence clones that have the correct length fragments (Basic Protocol 3) |