| 5X Phusion HF Buffer | 4 μl |
| 10mM dNTPs | 0.4 μl |
| oSQT 34 for TALENs or oSQT1 for TALE-activators (5 uM) | 1 μl |
| oSQT 35 for TALENs or oSQT38 for TALE-activators(5 uM) | 1 μl |
| Suspension of bacterial cells | 5 μl |
| Phusion | 0.2 μl |
| Nuclease free H20 | 8.4 μl |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.