| NEBuffer 4 | 25 μl |
| 100X BSA | 2.5 μl |
| XbaI (20 U/μl) | 5 μl |
| BamHI-HF (20 U/μl) | 5 μl |
| SalI-HF (20 U/μl) | 5 μl |
| Nuclease free H2O 107.5 μl |
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