(A) Experimental design to test the role of PR+ VMHvl neurons in male
behaviors.
(B–G) PRCre and control males were
injected with AAV-flex-taCasp3-TEVp and tested for mating, ultrasonic
vocalizations toward male or female intruders, and territory marking.
(B) PRCre males intromit females in fewer
assays.
(C, D) PRCre males mount and intromit
females less and have shorter bouts of intromissions.
(E) Both PRCre and control males emit more
vocalizations to females.
(F, G) No difference between PRCre and
control males in the number and distribution of urine
marks. % marks in center = 100*(# urine marks not
abutting cage perimeter/# of all urine marks).
Mean ± SEM; n ≥ 24/experimental group (B–D,
F,G), n ≥ 5/experimental group (E); *p < 0.008, **p <
0.001.
See also Figures S6
and S7.