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. 2013 Sep 9;8(9):e73268. doi: 10.1371/journal.pone.0073268

Figure 4. Bmi1 inhibits miR-200c expression via p53 downregulation.

Figure 4

(A) the inverse expression pattern between Bmi1 and miR-200c was showed; (B) p53 protein was downregulated in MCF-7/5-Fu cells, and pEGFP-N1-WTp53 transfection increased p53 protein in MCF-7 and MCF-7/5-Fu cells, respectively; (C) WTp53 upregulated miR-200c expression and restored Bmi1 mediated miR-200c downregulation; (D) GFP screen under fluorescence microscope to monitor ectopic WTp53 expression; (E) Ectopic expression of WTp53 upregulated miR-200c in MDA-MB-231 and MDA-MB-453 with mutational p53; (F) Bmi1 negatively regulated p53 protein expression; (G) coimmunoprecipitation showed Bmi1 interacted with p53 physically. All data was obtained from three independent experiments * p<0.05, * p<0.01.