Table 2. Total number of fragments, polymorphisms and taxon-specific fragments detected by AFLP analysis of all Leishmania specimens tested.
Group specific fixed private alleles (bp) | ||||||
Code | Selective primer combination (1) | Bands detected inL. panamensis | Polymorphism inL. panamensis (%) | L. panamensis | Subgenus Viannia | Subgenus Leishmania |
R10 | EcoRI-0/MseI-A | 99 | 89 | 159 | – | – |
R11 | EcoRI-0/MseI-C | 97 | 59 | 180, 312 | 64, 66, 116, 136, 141, 219, 270, 351, 399 | – |
R12 | EcoRI-0/MseI-G | 85 | 63 | 65, 183, 200, 341, 355, 373 | 66, 92, 136, 226, 250 | 120 |
R13 | EcoRI-0/MseI-T | 102 | 74 | 156, 356, 389 | 88, 205, 265 | - |
S9 | EcoRI-A/MseI-0 | 113 | 73 | 367 | 116, 216, 341 | – |
S12 | EcoRI-A/MseI-G | 79 | 64 | 67, 141, 154, 239 | 92, 104, 160, 165 | – |
S13 | EcoRI-A/MseI-T | 78 | 79 | 377, | 216 | 103 |
T9 | EcoRI-C/MseI-0 | 107 | 56 | 101, 183, 411, | 198, 219, 351 | 121 |
U9 | EcoRI-G/MseI-0 | 105 | 77 | 74, 85, 133, 134, 252 | 66, 135, 245 | 131, 170 |
V9 | EcoRI-T/MseI-0 | 75 | 49 | 88, 136, 144, 153, 210, 330 | 76, 99, 106, 162, 180 | – |
V13 | EcoRI-T/MseI-T | 71 | 78 | 155, 197, 208 | 100, 180 | – |
W13 | EcoRI-AA/MseI-T | 52 | 86 | – | 87, 128, 203 | – |
Z12 | EcoRI-AT/MseI-G | 41 | 70 | 170, 211 | 165 | – |
Total | 1104 | 70 |
(1) EcoRI: 5′-GACTGCGTACCAATTC-3′; MseI: 5′-GATGAGTCCTGAGTAA-3′.