Skip to main content
. 2013 Sep 9;8(9):e73177. doi: 10.1371/journal.pone.0073177

Table 2. Total number of fragments, polymorphisms and taxon-specific fragments detected by AFLP analysis of all Leishmania specimens tested.

Group specific fixed private alleles (bp)
Code Selective primer combination (1) Bands detected inL. panamensis Polymorphism inL. panamensis (%) L. panamensis Subgenus Viannia Subgenus Leishmania
R10 EcoRI-0/MseI-A 99 89 159
R11 EcoRI-0/MseI-C 97 59 180, 312 64, 66, 116, 136, 141, 219, 270, 351, 399
R12 EcoRI-0/MseI-G 85 63 65, 183, 200, 341, 355, 373 66, 92, 136, 226, 250 120
R13 EcoRI-0/MseI-T 102 74 156, 356, 389 88, 205, 265 -
S9 EcoRI-A/MseI-0 113 73 367 116, 216, 341
S12 EcoRI-A/MseI-G 79 64 67, 141, 154, 239 92, 104, 160, 165
S13 EcoRI-A/MseI-T 78 79 377, 216 103
T9 EcoRI-C/MseI-0 107 56 101, 183, 411, 198, 219, 351 121
U9 EcoRI-G/MseI-0 105 77 74, 85, 133, 134, 252 66, 135, 245 131, 170
V9 EcoRI-T/MseI-0 75 49 88, 136, 144, 153, 210, 330 76, 99, 106, 162, 180
V13 EcoRI-T/MseI-T 71 78 155, 197, 208 100, 180
W13 EcoRI-AA/MseI-T 52 86 87, 128, 203
Z12 EcoRI-AT/MseI-G 41 70 170, 211 165
Total 1104 70

(1) EcoRI: 5′-GACTGCGTACCAATTC-3′; MseI: 5′-GATGAGTCCTGAGTAA-3′.