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. 2013 Sep 9;8(9):e73483. doi: 10.1371/journal.pone.0073483

Figure 6. Impact of hypovirus infection on the morphology of Δprodh and Δp5cdh and on the accumulation of Prodh and P5Cdh transcripts in the wild-type strain.

Figure 6

A, Morphology of hypovirus-infected Δprodh and Δp5cdh mutants. The strains were cultured on PDA for 7 days; hypovirus was introduced into Δprodh and Δp5cdh from the hypovirus-infected strain EP713 by anastomosis. Paired inoculation of the Δprodh or Δp5cdh mutant with EP713 resulted in anastomosis and the conversion of the strains to the hypovirus infection phenotype. B, Transcript accumulation levels in hypovirus-free EP155 and hypovirus-infected EP713. Fungal strains were cultured on PDA at 25°C for 7 days, and mycelia were collected for mRNA isolation. The transcript levels were determined by RT-PCR using the Prodh-specific primer pair prodh-Qf/prodh-Qr and P5Cdh-specific primer pair p5cdh-Qf/p5cdh-Qr. The transcript level in EP713 is expressed as a percentage of the transcript level in EP155. The values were calculated from three independent experiments. The error bars represent standard deviations.