Figure 2. PNGS changes in different clade B Env quasispecies.

Regions of Envelope are noted in the top bar and below are the PNGS positions. All protein sequences were aligned to HXB2 (accession # AF033819) and were analyzed with the Aminotrack webserver (http://apps.sbri.org/AminoTrack/). 1: Presence of PNGS at a given amino acid position. Green boxes indicate the essential PNGS and purple boxes indicate the accessory PNGS for 2G12 recognition. 2G12 IC₅₀ (µg/mL) and the time post infection (PI) for the envelopes from the A141 macaque are indicated. Neutralization data and time since seroconversion (SC) for subjects ACH19545, ACH11686, ACH 18860 and ACH18839 from the Amsterdam cohort are reported per Quakkelaar et al. [24]. 2G12 IC₅₀ (µg/mL) and time since SC for subject ACH19542 from the Amsterdam cohort are adapted from Bunnik et al. [23]..