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. 2013 Sep 9;8(9):e75277. doi: 10.1371/journal.pone.0075277

Figure 3. Specific PNGS affect heterologous neutralization.

Figure 3

(A) Cartoon depicting PNGS profiles of A141 Env mutants. The mutants are indicated on the left and the corresponding PNG equivalent A141 clones are indicated on the right. Vertical bars indicate the presence of a PNGS at the amino acid positions (130, 139, 160 or 397) shown. (B) Serially diluted monoclonal antibodies (b12, 2G12, 4E10 and 2F5) were tested for the neutralization of the pseudovirus of interest [d56 B3, d417 D15, d487 A6-1 and d670 A8-9] or of the PNG mutant of interest [Δ(N130/397), Δ(N139/397) and Δ(N130/139/160/397)] in a TZM-bl cell assay. The MAb concentration (µg/mL) required to reach 50% neutralization of each pseudovirus is reported as IC50 (µg/mL). Data shown are mean +/- SD from three independent assays. P < 0.02 compared to d670 A8-9. # P = 0.0003 compared to d487 A6-1. $ P ≤ 0.0006 compared to d417 D15. P = 0.0042 compared to d56 B3.